Nonspecific Catalyses by α-Chymotrypsin and Trypsin

Trypsin and chymotrypsin inhibitors in insects and gut leeches.

The constant increase of life expectancy is associated with major ageing of developed populations. This indicates that the new century will have one of most epidemic progressions of cardiovascular, cancer and inflammatory diseases. The high challenge for medical research is to compress such morbidity. In these conditions, invertebrates have demonstrated to be truly useful models in drug discove...

Fate of trypsin and chymotrypsin in the human small intestine.

A trypsin and chymotrypsin inhibitor from chick peas (Cicer arietinum).

1. A trypsin and chymotrypsin inhibitor was isolated by extraction of chick-pea meal at pH8.3, followed by (NH4)2SO4 precipitation and successive column chromatography on CM-cellulose and calcium phosphate (hydroxyapatite). 2. The inhibitor was pure by polyacrylamide-gel and cellulose acetate electrophoresis and by isoelectric focusing in polyacrylamide gels. 3. The inhibitor had a molecular we...

Reactions of Trypsin and Chymotrypsin with Heparin, Trypsin Inhibitor, and Hexylresorcinol

il study of enzyme inhibitors designed to obtain information on the possible physiological significance of blood antiprotease has produced a number of simple fundamental reactions which have hitherto not been reported. It is the purpose of this paper to record these reactions for the possible benefit of others who may wish to investigate them further. Materials-The t,rypsin, chymotrypsin, and t...

Trypsin and chymotrypsin development in the neonatal lamb.

Twenty-two neonatal lambs were sacrificed at various ages (0, 1, 7, 14, 21, 28, 35 and 42 days postpartum), and assays for trypsin and chymotrypsin activity were conducted on the duodenal section of the small intestine. No trypsin activity was found at birth, but gradual nonsignificant increases in activity were found through day 7 and over a fourfold increase (P less than .01) was observed bet...